Probiotic activity of <i>Bacillus subtilis</i> metabolites in experimentally induced dysbiosis in mice

Scientific relevance. A promising option for dysbiosis correction is the use of metabiotics, products based on metabolites probiotic microorganisms. During fermentation, Bacillus subtilis bacteria (strains 3H and 1719) produce that exhibit properties in vitro . These observations motivate an vivo investigation B. metabolite effects colonic mucosal microbiota mice experimentally induced assessment potential as metabiotics. Aim. The authors aimed to compare activity 1719 a commercial metabiotic antibiotic-induced mice. Materials methods. experimental BALB/c weighing 18–20 g by intraperitoneal injection gentamicin. For subsequent correction, test groups received sorbent-bound metabolites, comparison group containing (VKPM B-2335(3)3) via intragastric 21 days. quantitative qualitative analysis included microbial culturing colony identification MALDI-TOF mass spectrometry. Results. Antibiotic-induced manifested itself decrease dominant increase opportunistic pathogens. After 7 days administration, Lactobacillus population returned normal all treatment groups. showed best results: their spp. composition corresponded intact animals. content Lac + Escherichia coli 100% observed elimination (Rodentibacter spp., Aerococcus spp.) fungi ( Trichosporon Kazachstania group; (no effect ; Enterococcus Rodentibacter group. Conclusions. Metabolites strains help restore diversity abundance dysbiosis. differences re-establishment indicate there interstrain variability metabolites.